MORINGA LEAF EXTRACT IMPROVES SPERMATOZOA MOTILITY AND VIABILITY THROUGH AN ANTIOXIDANT MECHANISM IN OLD WISTAR RATS

Reproductive aging is characterized by a progressive decline in physiological integrity that triggers damage to the testes, epididymis and other reproductive organs. Oxidative stress is associated with aging and infertility in men which causes a decrease in semen volume, abnormal morphology, decreased sperm viability and motility.  One of the treatments for oxidative stress is the use of antioxidant compounds such as Moringa plants. The purpose of this study was to determine the antioxidant mechanism of moringa leaf ethanol extract on sperm motility and viability in old Wistar rats.This type of research is randomized post-test only control group design. The total sample was 36 old wistar rats, which were randomized into 2 groups, the treatment group (18 rats) was given ethanol extract Moringa oleifera (EEMO) 50 mg/kg BW + CMC 0.5%, 0.5 ml / day. The control group (18 rats) was given CMC 0.5%, 0.5 ml/day, for 30 days. Termination of rats using the cervical dislocation method. Motility and viability of spermatozoa were examined. Data analysis using independent sample t-test and Mann Whitney with a significance value of p = 0.05.The results showed that the antioxidant content of EEMO 50 mg/kg BW + CMC 0.5%, for 30 days in old wistar rats increased progressive motility, decreased non-progressive motility and immotility, and increased spermatozoa viability, significantly in the treatment group compared to the control group with a p value 0.05. In conclusion, Moringa Leaf Extract Improves Spermatozoa Motility and viability through an antioxidant mechanism in old wistar rats.