Fatty acid profiles recorded in ocean prey and California salmonine eggs reveal maternal ocean diets linked to thiamine deficiency

Abstract Following the principle ‘you are what you eat’, fatty acid signatures (FASs) of adult fish tissues, when compared with those of potential prey, can link diet to nutritional status. In 2020, thiamine (vitamin B1) deficiency was diagnosed for the first time in multiple anadromous salmon populations in California. However, direct information linking ocean diet to thiamine status in marine-feeding salmonines is typically unavailable. This research aimed to link diet composition and egg total thiamine concentration of anadromous Chinook salmon, coho salmon and steelhead using FAS analysis. From 2020 to 2022, unfertilized eggs from these California salmonine species were collected alongside key forage species from the Pacific Ocean. FASs in eggs and prey were quantified using gas chromatography/mass spectrometry and total thiamine concentrations were quantified using high-performance liquid chromatography. Eggs rich in oleic acid (18:1n-9) reflected a diet dominated by Pacific herring and exhibited higher total thiamine concentrations. Conversely, eggs rich in eicosapentaenoic acid (20:5n-3) indicated a diet dominated by northern anchovy or euphausiid krill and were more likely to have low total thiamine concentrations. Polyunsaturated fatty acid proportions and the egg unsaturation index were strongly negatively correlated with egg total thiamine concentration, emphasizing the importance of lipid quality over quantity as a driver of egg thiamine concentration. This study highlights the utility of FASs for tracking the impacts of a changing ocean prey base on diet and underscores how dietary shifts can directly impact egg thiamine status in anadromous salmonines.