MiR-335-3p Alleviates Adipogenesis and Inflammation in Bone Marrow-Derived Mesenchymal Stem Cells in Osteoporotic Fractures.

Osteoporotic fractures (OPF) represent a substantial share of all fracture types, seriously affecting the quality of life. Therefore, it is essential to find a biomarker for early diagnosis and prevention of OPF. Detection of mRNA expression was performed using RT-qPCR. The diagnostic value of miR-335-3p in OPF was examined by the ROC curve. Pearson's correlation analysis was employed to explore the correlation. The rat model of OPF was established by bilateral oophorectomy (OVX) and creation of a fracture in the middle of the left femur. Then, bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the rats. MiR-335-3p was over-expressed or inhibited by transfection in BMSCs. Adipogenic factors, inflammatory factors, osteogenic markers and cell metabolic activity were detected in BMSCs. MiR-335-3p expression was markedly reduced in patients with OPF, and this molecule exhibited favorable diagnostic efficacy for OPF. In OPF, miR-335-3p was positively correlated with bone mineral density. In BMSCOPF, miR-335-3p reduced inflammatory factors and increased cell metabolic activity. In addition, the over-expression of miR-335-3p reduced adipogenic factors and increased osteogenic markers. In conclusion, miR-335-3p was decreased in OPF and may have high diagnostic potential in OPF. In BMSCOPF, miR-335-3p is downregulated in OPF and possesses promising diagnostic value. It can inhibit adipogenic differentiation and inflammatory responses while promoting osteogenic differentiation in BMSCs. These findings suggest that miR-335-3p may participate in the pathogenesis of OPF and be associated with fracture healing.