DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISM USING TARMS-PCR IN TLR2 GENE IN LOCAL CATTLE OF JAMMU

The local cattle of Jammu is one of the unique cattle which belongs to hilly areas of Jammu. A major group of Pattern recognition receptors (PRRs) are the Toll like receptors (TLRs) which play a very important role in immunity of the animal. Therefore, the study was aimed to detect the polymorphism in TLR2 gene. The genomic DNA was isolated from blood samples of 50 unrelated local cattle of Jammu from different locations.Tetra primer amplification refractory mutation system- polymerase chain reaction (TARMS-PCR) using self-designed primers was performed to detect the SNPs. TARMS-PCR for TLR2 revealed three bands on gel (158 bp control fragment, 120 bp B allele, 100 bp A allele). The genotypic frequencies for AA, AB and BB genotypes and allele frequency of A and B alleles were 0.12, 0.64 and 0.24, 0.44 and 0.56, respectively. The calculated observed number of alleles (na ), effective number of alleles (ne ), observed homozygosity (Ho ), expected heterozygosity (He ), Shannon’s information index (I),Wright’sfixation index (FIS ), PIC values were 2.00, 1.9716, 0.5022, 0.497, 0.6859,-0.2987, 0.3713, respectively. The χ2 and G2 values for genotypes were significant. From the present study it can be concluded that there are substantial genetic variations and heterozygosity present within the local cattle population of Jammu province, which can be explored to formulate the effective breeding policies for genetic improvement, propagation and conservation of this important local cattle population.