BiologyChemistryMedicine

Sensitive and Robust One-Pot RPA-CRISPR/Cas12a Assay with Elimination of cis-Cleavage.

Ruiying Han, Ning Xiao, Zhirui Wu, Yingying Zhao, Xiaoli Wang, Xiaochuan Tang

2026.5.12ANALYTICAL CHEMISTRY

DOI: 10.1021/acs.analchem.5c06674

Abstract

and Avian Leukosis Virus subgroup J (ALV-J) samples. Overall, the 11bp+9ss one-pot assay exhibits superior sensitivity and robustness, showing great potential for POCT of bacteria and viruses.

Citation format

HAN, Ruiying, et al. Sensitive and robust one-pot RPA-CRISPR/Cas12a assay with elimination of cis-cleavage. ANALYTICAL CHEMISTRY, 2026, 98 18: 13307–13318.