Development of Saccharomyces cerevisiae isobutanol production strain from osmotolerant and ethanol-producing industrial isolated yeast

Highlights • Isobutanol-producing S. cerevisiae strain for industrial application.• Isobutanol-tolerant strain developed from conventional mutagenesis.• CRISPR-Cas9-mediated BAT1 knockout to enhance isobutanol production.• CRISPR-Cas9 avoids the presence of plasmids and antibiotic markers.• Strain highly increased isobutanol yield, potential for bio-alcohol production.