Upregulation of Glucomannan Synthesis Genes in Porang (Amorphophallus muelleri Blume) after Polyploidy Induction using Colchicine

Inducing polyploidy in porang using colchicine has been explored and successfully enhanced the genetic variability of porang. However, the specific impact of poly- ploidy on the expression of the genes responsible for glucomannan synthesis in porang has not been extensively studied. Therefore, this study aims to evaluate the effect of polyploidy induction on the expression of genes responsible for glucoman- nan synthesis in porang. Porang shoot explants (2n = 26) were used for polyploidy induction. Polyploidy induction was carried out using colchicine added to the cul- ture medium. The concentrations of colchicine used in this study were 0 %, 0.01 %, 0.02 %, 0.03 %, and 0.04 %. After 53 days in media without colchicine, explants were collected for flow cytometry analysis and RNA isolation. The application of colchicine at various concentrations induced tetraploid and mixoploid porang. Mu- tation induction using colchicine also increases the expression (upregulation) of the CSLA, GMPP, CSLD, and GMPP genes encoding glucomannan biosynthesis in porang. Tetraploid (0.01 % colchicine treatment) and mixoploid porang (0.02 % colchicine treatment) showed the highest expression of genes involved in gluco- mannan biosynthesis via both sucrose and invertase pathways. Copyright: © 2026, J. Tropical Biodiversity Biotechnology (CC BY-SA 4.0) Research Article Upregulation of Glucomannan Synthesis Genes in Porang (Amorphophallus muelleri Blume) after Polyploidy Induction using Colchicine Didik Wahyudi1*, Suyono1, Ruri Siti Resmisari2, Nida Annisa Sholeha2, Sabrina Labista Wibowo2, Devi Rahmawati Kafara2 1) Plant Physiology Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia 2) Plant Tissue Culture Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia * Corresponding author, email: didik_wahyudi@bio.uin-malang.ac.id Journal of Tropical Biodiversity and Biotechnology Volume 11, Issue 01 (2026): jtbb19849 DOI: 10.22146/jtbb.19849