Fabrication of Si and Mg-infused alginate hydrogel membranes for hemostasis and periodontal regeneration

Introduction: Periodontal disease is a prevalent oral health issue that leads to the deterioration of periodontal tissues, ultimately resulting in tooth loss. Periodontal regeneration techniques, such as guided tissue regeneration (GTR), have gained enormous attention for their potentiality to restore damaged periodontal tissues. In this study, fabricated alginate hydrogel membranes with silica and magnesium nanoparticles are proposed to enhance the hemostasis as well as mimics the periodontal regeneration. Materials and Methods: Sodium alginate (3%) polymeric solution was prepared. In the prepared polymer solution, silica (100 mg) and magnesium (100 mg) were suspended, respectively. Then, subsequently, metal oxide (Si and Mg) was included in the polymeric matrix and blended well for appropriate mixing. Further, these alginate polymers were cross-linked with calcium chloride to fabricate metal nanoparticulates infused alginate membranes. Results: The results of this study demonstrate the successful fabrication of polymeric hydrogel membranes reinforced with silica and magnesium. Crystalline properties (SiO 2 and MgO, Mg(OH) 2 , as well as Mg-oxalate) authenticate the formation of nanoparticles with the relevant morphological features. The combination of silica and magnesium particles in the membrane showed elevated blood compatibility with hemostatic properties. Conclusion: In conclusion, silica and magnesium-reinforced polymeric hydrogel membranes show promising results for compatibility and hemostasis, which supports GTR in periodontal therapy. These membranes have the potentiality to improve the outcomes of periodontal defect treatments and contribute to the advancement of regenerative dentistry. Silica is a component of the extracellular matrix, and both silica and magnesium are involved in the synthesis of collagen that supports for wound healing. Hemostatic material demand in periodontal treatment is essential and also plays a pivotal role to control bleeding as well as in regeneration.