Fuorescent dye diffusion coefficient measurement in murine brain tissues using the method of fluorescence recovery after photobleaching

Background and Objectives: The molecular transport in the brain tissue is yet to be fully studied and presents great interest to the researchers in the fields of biophysics and biomedicine. Diffusion and molecular transport processes in brain tissues are defined by the properties of the intercellular space of the central nervous system. The object of this work is to develop a method for ex vivo quantitative measurements of diffusion in the brain tissues of laboratory mice. Materials and Methods: Multiphotonmicroscopy-based fluorescence recovery after photobleaching was applied in the analysis of fluorescent dye molecular mobility dynamics in mouse brain tissue. Results: The results of the fluorescence recovery dynamics analysis in an ex vivo slab of the mouse brain have been used for molecular diffusion mapping in a layer of brain tissues of a laboratory mouse. Diffusion coefficients in several sections of the layer of brain tissues have been calculated based on a simplified diffusion model and fluorescence recovery after photobleaching data. Conclusion: Fluorescence recovery after photobleaching with multiphoton microscopy are applicable in the creation of the molecular diffusion map in the brain tissue of a laboratory mouse.