Detection of a blaKPC-2 carrying IncP-6 plasmid in Aeromonas caviae isolated from a clinical sample in a Spanish hospital

The emergence of carbapenemase-producing Aeromonas species represents a growing concern due to their potential role as reservoirs and disseminators of antimicrobial resistance genes in aquatic and clinical environments. In this study, we describe the genomic characterization of a clinical Aeromonas caviae isolate harboring a plasmid-encoded KPC-type carbapenemase. This microorganism was isolated from a stool sample from a patient with acute gastroenteritis in May 2025. Whole-genome sequencing followed by bioinformatic analysis using ResFinder, PlasmidFinder, and MobileElementFinder revealed the presence of the bla KPC-2 gene located on an IncP-6 plasmid. The plasmid carried multiple insertion sequences and composite transposons, including IS26, ISKpn3, and IS6100, suggesting a highly mobile genetic platform. Comparative analysis indicates that the plasmid structure resembles previously reported KPC-harboring plasmids found in Enterobacterales, highlighting potential interspecies transfer events. To our knowledge, this is one of the few reports describing an IncP-6 plasmid carrying bla KPC-2 in Aeromonas caviae. These findings underscore the importance of environmental and clinical surveillance of carbapenemase-producing bacteria.