Analysis of Six Sugars in Domestically Distributed Seeds and Mushrooms

This study analyzed sugars in 67 varieties of seeds and mushrooms to generate reliable data for the Korean Food Composition Table (KFCT). High performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) was utilized as a sensitive and selective method, offering superior resolution compared with conventional approaches. Six sugars were evaluated, including fructose, glucose, sucrose, lactose, maltose, and galactose. The coefficients of determination ( R 2 ) for calibration curves of all sugars were above 0.9995, with detection limits ranging from 5.34 to 37.50 mg/L and quantitation limits from 17.79 to 125.00 mg/L. Accuracy was validated using Standard Reference Material (SRM) 3233, showing recoveries ranging from 82.92% to 104.57% and repeatability between 0.27% and 1.80%. Among the analyzed samples, fructose, galactose, glucose, sucrose, and maltose were commonly detected. However, lactose was not detected in any samples. Variations in sugar composition were observed across food categories, suggesting the effects of processing