Semi‐Automated and Consistent Fabrication Method of ssDNA‐Functionalized Single‐Walled Carbon Nanotube Biosensor

Single-walled carbon nanotubes (SWCNTs) exhibit exceptional optical and electronic properties but require proper dispersion in a liquid for solution process and biological applications. Here, we present a semi-automated platform for consistent fabrication of ssDNA-wrapped SWCNT dispersions. Conventional manual preparation methods suffer from batch-to-batch variations due to imprecise SWCNT weighing and inconsistent sonication parameters. Our system integrates an XYZ-motorized stage with a programmable tip sonicator and the preparation of SWCNT mass source with high consistency, enabling unprecedented consistency and automated processing of multiple samples. This platform enables systematic investigation of fabrication parameters, including ssDNA:SWCNT mass ratio, sonication power, and duration. We evaluate the performance of these hybrids as serotonin nanosensors, analyzing their fluorescence response characteristics and sensitivity. Our approach significantly reduces human labor while providing precise control over critical parameters, yielding consistent, high-quality serotonin-responsive nanosensors. This research demonstrates the importance of automated fabrication processes for achieving reliable and scalable production of SWCNT-based nanomaterials with uniform sensing properties.