Microcirculation parameters in rats exposed to biotechnological preparations of Cordyceps militaris fungi

BACKGROUND: Our previous studies demonstrated that administration of Cordyceps militaris (L.) Fr. biomass and culture fluid extracts resulted in significant changes in blood pressure and heart rate in rats. However, the effect of these fungal biotechnological products on microcirculation processes has not been studied. BACKGROUND: Our previous studies demonstrated that administration of Cordyceps militaris (L.) Fr. biomass and culture fluid extracts resulted in significant changes in blood pressure and heart rate in rats. However, the effect of these fungal biotechnological products on microcirculation processes has not been studied. AIM: To determine changes in microcirculation parameters in rats exposed to Cordyceps militaris (L.) Fr. mushroom extracts. METHODS: Group 1 served as a control group, Group 2 (ECG) received 0.5 ml of C. militaris culture fluid extract, and Group 3 (EBM) received 0.5 ml of Cordyceps militaris (L.) Fr. biomass extract. Microcirculation was studied using laser Doppler flowmetry using a Lazma-MC laser blood flow analyzer (version 2, manufactured by Lazma Research and Production Enterprise, Russia). Both non-oscillatory and oscillatory parameters of tissue microhemodynamics were recorded. RESULTS: Intraperitoneal administration of an extract of the in vitro-obtained mycelial biomass (EBM) of Cordyceps militaris (L.) Fr. resulted in a 30.3% increase in tissue perfusion (p=0.0026) due to increased endothelial metabolic activity, decreased sympathetic adrenergic pressor effects, and decreased precapillary sphincter and precapillary metarteriole tone due to the development of Ca2+-dependent muscle relaxation. This was evidenced by significant increases in all oscillatory and non-oscillatory parameters of microcirculation. With the administration of ECG Cordyceps militaris (L.) Fr., perfusion increased by 32.6% (p=0.0016), Ae - by 55% (p=0.0072), An - by 77.9% (p=0.0121), Am by - 82.1% (p=0.0099), and Ad - by 45.5% (p=0.0370) compared to those in the control group of animals, indicating the development of vasodilation. CONCLUSIONS: Thus, as analysis of the study results showed, the administration of C. militaris ECF and EBM to animals has a beneficial effect. led to an increase in endothelium-dependent vasodilation, a decrease in peripheral resistance, an increase in blood flow into the nutritional microvascular bed, and an improvement in venous outflow.