Design and optimization of diacerein loaded lipid nanocarriers: Characterization, in vitro and in vivo evaluation for anti-arthritic activity.

Solid lipid nanoparticles (SLNPs) are recognised as dependable substitute for conventional polymeric nanocarriers for lipophilic drugs. Here, diacerein (DC) (BCS class II drug) loaded SLNPs were developed via solvent evaporation technique for enhancing the in vitro dissolution of DC and optimize (32 full factorial design) for representing the effect of independent parameters on dependent factors following response surface methodology. Physico-chemical parameters, DSC, XRD, microscopic analysis, and stability were assessed. Optimized DC-SLNPs showed nano size (303 ± 0.78 nm), encapsulation efficiency (79.5 ± 0.5%) and DC content (88.59 ± 0.6%). The dose dependent lowering of cell viability and anti-inflammatory response to lipopolysaccharide (LPS)-induced stress in RAW 264.7 cells were studied. DC-SLNPs resulted in optimal protection against LPS-induced stress (1 μg/ml) when compared with DC. These were observed in optimized DC loaded SLNPs in comparison to DC·In vivo anti-arthritic activity and satisfactory percentage bias (1.32-3.15) suggested the potentiality of DC-SLNPs.