Protective Effect of Chlorogenic Acid on endoplasmic reticulum (ER) stress and Oxidative Stress Crosstalk in Mice

10.30699/jambr.33.157.157 Background & Objective: Cross-talk between endoplasmic reticulum (ER) stress and oxidative stress has been recognized as contributing to several pathological conditions, particularly those affecting the respiratory system. This study aims to investigate the potential protective role of chlorogenic acid (CA) against oxidative stress in a murine model of ER stress induced by tunicamycin. Materials & Methods: t 36 male C57BL/6 mice were divided into six groups: a control group (saline), a vehicle group (DMSO), a CA group, a tunicamycin group, a CA + tunicamycin group, and a CA + tunicamycin group (both with a 20 mg/kg CA dose). After 72 hours of treatment, protein levels were investigated by ELISA, mRNA levels by RT-PCR, and histopathological alterations were examined by H&E staining. Results: We found that 20 mg/kg CA alleviated GRP78 mRNA levels, decreased MDA, and increased GSH levels. It also showed potent anti-inflammatory and anti-fibrotic effects in the lungs of ER-stress induced mice. Conclusion: Chlorogenic acid administration decreased oxidative stress markers by alleviating ER stress. This indicates a mechanistic link between ER stress and oxidative stress.