Expression of fimA and csgA genes in uropathogenic Escherichia coli treated with supernatant of Lactobacillus plantarum plus gentamicin

A total of 422 patients with type 2 diabetes mellitus (T2DM) were analysed via standard microbiological techniques to detect the presence of Escherichia coli. This study aimed to assess UPEC isolates' adhesion to urothelial cells and their ability to produce haemolysin via sheep blood agar. The biofilm formation of E. coli isolates was investigated. The impact of each antibiotic, both individually and in combination, on biofilm gene expression was investigated. A total of 26.19% of the isolates were identified as E. coli. The minimum inhibitory concentration (MIC) for the CFS of Lactobacillus was 12.5 U/ml. The results indicated that all strains of Escherichia coli exhibited a 50% success rate in sticking to epithelial cells, and all isolates demonstrated the ability to form biofilms. In response to the combination of CFS and antibiotics, RT-PCR analysis revealed significant suppression of biofilm-producing genes (fimA and csgA) compared to those in response to monotherapy.