BiologyEnvironmental ScienceMedicine

D. Marie, S. Brown

1993BIOLOGY OF THE CELL

DOI: 10.1016/0248-4900(93)90113-s

tlooto Summary

An extensive graphical guide to interpreting DNA histograms and their problems is given, and cytometry laboratories unfamiliar with plant sciences will find herein a guide, and references, to adapt their methods to plant material.

Abstract

Summary— An introduction is given to the literature concerning methods and objectives for cytometric DNA analysis of plant nuclei. This area has gained relevance with applications in plant breeding and seed production industries, where laboratories unfamiliar with cytometry are adopting the method. An extensive graphical guide to interpreting DNA histograms and their problems is given. Conversely, cytometry laboratories unfamiliar with plant sciences will find herein a guide, and references, to adapt their methods to plant material. A table of 2C values reassessed by flow cytometry for 70 plant species, plus the genome composition (GC%) in many instances, is also included.

Citation format

MARIE, D.; BROWN, S. A cytometric exercise in plant DNA histograms, with 2c values for 70 species. BIOLOGY OF THE CELL, 1993, 78.