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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

C. Yanisch-Perron, J. Vieira, J. Messing

1985GENE

DOI: 10.1016/0378-1119(85)90120-9

tlooto Summary

New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.

Abstract

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Citation format

YANISCH-PERRON, C.; VIEIRA, J.; MESSING, J. Improved m13 phage cloning vectors and host strains: Nucleotide sequences of the m13mp18 and puc19 vectors. GENE, 1985, 33 1: 103–19.