Acceso abiertoEnvironmental ScienceBiologyMedicine

Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis.

F. Wintzingerode, U. Göbel, E. Stackebrandt

1997.11.1FEMS MICROBIOLOGY REVIEWS

DOI: 10.1111/j.1574-6976.1997.tb00351.x

Resumen de tlooto

Specific aspects of sample collection, cell lysis, nucleic acid extraction, PCR amplification, separation of amplified DNA, application of nucleic probes and data analysis are covered.

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Formato de cita

WINTZINGERODE, F.; GÖBEL, U.; STACKEBRANDT, E. Determination of microbial diversity in environmental samples: Pitfalls of PCR-based rrna analysis. FEMS MICROBIOLOGY REVIEWS, 1997, 21 3: 213–29.