Affordable image analysis using NIH Image/ImageJ.

CMYK47 Sir, The contribution of scientific technology in the field of histopathology and lab medicine is well recognized. Image analysis is one such division in which many researchers and practicing pathologists have used scientific technology to good advantage. Currently there are many systems available to an anatomic pathologist for the purpose of image analysis in immunohistochemistry (IHC) and for nuclear morphometry. Many of these systems, however, require expensive software and hardware attachments for image acquisition, analysis and storage. Consequently, a costeffective alternative for image analysis would be a welcome tool for pathologists and researchers alike. In this connection, “ImageJ” is a freely available java-based public-domain image processing and analysis program developed at the National Institutes of Health (NIH). Currently, ImageJ’s Macintosh platform counterpart, “NIH Image” is widely used in biologic research.