Open AccessBiologyMedicine

Real-time RT-PCR normalisation; strategies and considerations

J. Huggett, K. Dheda, S. Bustin, Alimuddin Zumla

2005.6.1GENES AND IMMUNITY

DOI: 10.1038/sj.gene.6364190

tlooto Summary

This review discusses the relative merits of different normalisation strategies, suggests a method of validation that will enable the measurement of biologically meaningful results and discusses the popular practice of measuring an internal reference or housekeeping gene.

Abstract

Abstract is not available.

Citation format

HUGGETT, J., et al. Real-time RT-PCR normalisation; strategies and considerations. GENES AND IMMUNITY, 2005, 6: 279–284.