B. Bill, Andrew M. Petzold, K. Clark, L. Schimmenti, S. Ekker
2009.3.1Zebrafish
tlooto Summary
Common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system are discussed and new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.
Abstract
Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.
Citation format
BILL, B., et al. A primer for morpholino use in zebrafish. Zebrafish, 2009, 6: 69–77.