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BiologyMedicine

A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.

R. Dale, B. McClure, J. P. Houchins

1985PLASMID

DOI: 10.1016/0147-619x(85)90053-8

tlooto Summary

A simple new procedure was described for producing a sequential series of overlapping clones for use in DNA sequencing by using single-stranded M13 DNA and complementary DNA oligomers to form specific cleavage and ligation substrates.

Abstract

Abstract is not available.

Citation format

DALE, R.; MCCLURE, B.; HOUCHINS, J. P. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18 s rdna. PLASMID, 1985, 13 1(1): 31–40.