J. Chirgwin, A. Przybyla, Raymond J MacDonald, W. Rutter
1979.11.27BIOCHEMISTRY
tlooto Summary
Intact ribonucleic acid has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds.
Abstract
Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was isolated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of alpha-amylase messenger ribonucleic acid.
Citation format
CHIRGWIN, J., et al. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. BIOCHEMISTRY, 1979, 18 24: 5294–9.