BiologyMedicine

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

F. Bolivar, Raymond L. Rodriguez, Patricia J. Greene, M. Betlach, H. Heyneker, H. W. Boyer, J. H. Crosa, S. Falkow

1977.11.1GENE

DOI: 10.1016/0378-1119(77)90000-2

tlooto Summary

In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).

Abstract

Abstract is not available.

Citation format

BOLIVAR, F., et al. Construction and characterization of new cloning vehicles. II. a multipurpose cloning system. GENE, 1977, 2 2: 95–113.