Prevalence of sub-clinical mastitis among dairy cattle in Bhaktapur District, Nepal.

*Corresponding Address: shreanstha@yahoo.com The study was conducted to estimate the prevalence of subclinical mastitis (SCM) in dairy cows in the Bhaktapur District of Nepal. A total of 200 milk samples were collected from 50 dairy cows. The California mastitis test (CMT) was carried out on quarter milk samples to determine the prevalence of subclinical mastitis. California Mastitis Test (CMT) of these samples revealed that 52 % of animal suffered from SCM. The samples positive to CMT were cultured in Nutrient, MacConkey and blood agar. The bacteria isolated were Staphylococcus spp. (50 %), Streptococcus spp. (27 %), Escherichia coli (10 %), Corynebacterium (8 %), Salmonella (4 %) and Enterobacter spp. (1 %).