T. Nakagawa, Takamasa Suzuki, Satoko Murata, Shinya Nakamura, Takeshi Hino, K. Maeo, Ryo Tabata, T. Kawai, Katsunori Tanaka, Y. Niwa, Yuichiro Watanabe, Kenzo Nakamura, Tetsuya Kimura, S. Ishiguro
tlooto Summary
Improved pGWBs should be a powerful tool for transgenic research in plants because of their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing.
Abstract
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6×His, FLAG, 3×HA, 4×Myc, 10×Myc, GST, T7, and TAP, were employed. Some vectors carry the 2×35S-Ω promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.
Citation format
NAKAGAWA, T., et al. Improved gateway binary vectors: High-performance vectors for creation of fusion constructs in transgenic analysis of plants. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2007, 71: 2095–2100.