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EngineeringBiologyMedicine

Genome engineering using CRISPR-Cas9 system.

Le Cong, Feng Zhang

2015Methods in Molecular Biology

DOI: 10.1007/978-1-4939-1862-1_10

tlooto Summary

This chapter presents all relevant methods including the initial site selection, molecular cloning, delivery of guide RNAs and Cas9 into mammalian cells, verification of target cleavage, and assays for detecting genomic modification including indels and homologous recombination.

Abstract

Abstract is not available.

Citation format

CONG, Le; ZHANG, Feng. Genome engineering using CRISPR-Cas9 system. Methods in Molecular Biology, 2015, 1239: 197–217.