ChemistryBiologyMedicine

Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.

J. Arnau, C. Lauritzen, G. Petersen, J. Pedersen

2006.7.1PROTEIN EXPRESSION AND PURIFICATION

DOI: 10.1016/j.pep.2005.12.002

tlooto Summary

A survey of the most common affinity tags and the current methods for tag removal is presented, with special emphasis on the removal of N-terminal histidine tags using TAGZyme, a system based on exopeptidase cleavage.

Abstract

Abstract is not available.

Citation format

ARNAU, J., et al. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. PROTEIN EXPRESSION AND PURIFICATION, 2006, 48 1: 1–13.