Oxymatrine induced apoptosis in multiple myeloma cell line U266 and its mechanism

Objective:To evaluate the apoptosis of multiple myeloma(MM)cell line U266 induced by oxymatrine and its mechanism.Method:MM cell line U266 were treated with different concentrations(0,5,10or20mmol/L)of oxymatrine.The cell proliferation,cell apoptotic morphology,cell apoptosis and protein expression of apoptosis factors(Bax and Bcl-2)were respectively determined by MTT method,Wright staining,flow cytometry and western blot.Result:Oxymatrine(5,10 or 20 mmol/L)significantly inhibited the cell proliferation of U266,and showed a dose dependent effect.After treatment of 20mmol/L oxymatrine for 48 h,U266showed typical apoptotic morphological changes.After treated with oxymatrine(5,10 or 20mmol/L)for 48 h,the apoptosis rate of U266 was respectively(11.5±1.4)%,(24.7±2.5)% and(43.6±3.4)%,and showed a dose dependent effect.After U266 were treated with oxymatrine(5,10 or 20 mmol/L)for 48 h,the expression of Bax increased and Bcl-2decreased,both dose dependently.Conclusion:Oxymatrine can induce the apoptosis of MM cell line U266.Regulating the expression of apoptosis related gene Bax and Bcl-2may be its mechanism.