Relationship Between Apolipoprotein A5-1131T＞C Polymorphism and Coronary Heart Disease

Objective:To investigate the correlation between apolipoprotein (APO) A5-1131TC polymorphism and coronary heart disease (CHD). Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polyacrylamide gel eletro-phoresis (PAGE) were used to analyze genotypic polymorphism in 186 patients with CHD and 268 healthy controls. The levels of serum lipid profiles were also measured by enzymatic methods. Results:The APOA5-1131T C genotype distribution in CHD group and the control group showed a significant difference. The frequency of the -1131C allele in patients with CHD was significantly higher than that of the controls (0.422 vs. 0. 321 ,P0.05). The serum TG levels among three genotype groups showed a significant difference. The TG levels in type TC( 1. 50±0.78 mmol/L) and type CC( 1.73±1.22 mmol/L)were significantly higher than in type TT(1. 29±0. 74 mmol/L) (all P0.05), but there was no significant difference between type TC and type CC(P0.05). Compared with noncarriers (type TC +CC), C carriers (type TT) also had a higher TG levels in CHD group (1.46±0. 86 mmol/L vs 1. 73±0. 95 mmol/L, P 0. 05). The levels of TC, HDL-C and LDL-C showed no statistical differences between various genotypes (P 0.05). Unadjusted logistic regression analysis indicated that TC + CC genotype of A5-1131TC had an increased CHD risk (OR = 1. 520,95% CI 1. 044-2. 215,P =0. 029). But the analysis did not show that TC + CC genotype was independently associated with an increased risk of CHD (OR = 1. 331,95% CI 0. 634-2. 794, P= 0.449) after adjusted for BMI, presence of hypertension or diabetes and HDL-C levels. Conclusion; The frequency of APOA5-1131C allele in patients with CHD was significantly higher than that of the healthy individuals. The APOA5-1131T C variant was significantly associated with increased serum TG levels. The APOA5-1131T C variant may be associated with an increased risk of CHD. However, as an independent risk factor for CHD needs further study.