Surveillance of antibiotic resistance in the gram-negative bacteria isolated from hospitals in Qingpu,Shanghai during 2013

Objective To investigate the antibiotic resistance in clinical strains of gram-negative bacteria isolated from the hospitals in Qingpu District from January through December during 2013.Methods Antimicobial susceptibility testing was carried out for the gram-negative isolates according to an agreed protocol using automated system.Results were interpreted according to CLSI 2013 breakpoints.Results A total of 1 376 clinical isolates were included in this analysis.The overall prevalence of ESBLs-producing srains was 66.7%in E.coli,28.7%in Klebsiellaspp.and 12.5%in P.mirabilis.ESBLpositive strains were more resistant to antimicrobial agents than ESBL-negative strains.Enterobacteriaceae strains were still highly susceptible to carbapenem antibiotics.Overall,4.2% and 6.8% of the Enterobacteriaceae strains were resistant to imipenem and amikacin,respectively.About 17.1% of the P.aeruginosastrains and 36.0% of the Acinetobacterspp.strains were resistant to imipenem.A few extensively drug-resistant strains were identified in E.coli,K.pneumoniae,A.baumannii and P.aeruginosa.Conclusions Antibicotic resistance is a serious threat to clinical practice.Management and control of extensively drug-resistant bacteria is a great concern of clinicians.