The protective effect of Sacchromyces boulardii on intestinal mucosal barrier of rats with cirrhosis

Objective To investigate the protective effect and possible mechanism of Sacchromyces boulardii on intestinal mucosal barrier of rats with cirrhosis. Methods The rat models of cirrhosis were made by administering40% carbon tetrachloride and 10% ethanol solution. After 12 weeks,Sacchromyces boulardii was administered to rats in treatment group 75 × 108 CFU /( kg·d) by gastric irrigation for 14 days,while the control group and model group were given equivalent dose of saline. The endpoint chromo-genic TAL assay was used to detect plasma endotoxin levels,spectrophotometry method to determine the activity of diamine oxidase( DAO) in intestinal tissue,and automatic biochemical analyzer to detect serum ALT and AST levels. Results The concentration of endotoxin in the model group( 0. 251 ± 0. 011) was significantly higher than that in the treatment group( 0. 168 ± 0. 012)( P 0. 01). The activity of DAO in the model group( 0. 432 ± 0. 074) was significantly lower than that in the control group( 0. 728 ± 0. 065)( P 0. 01),and that in the treatment group( 0. 548 ± 0. 053) was significantly higher than that in the model group( P 0. 01); the difference between the treatment group and the control group was statistically significant( P 0. 01). The concentrations of serum ALT and AST in model group( 133. 89 ± 8. 09,176. 92 ± 10. 94) were significantly higher than those in the control group( 36. 73 ± 6. 95,41. 35 ± 10. 07)( P 0. 01),and those in treatment group were significantly lower than those in the model group( P 0. 01). Conclusion The intestinal mucosa of rats with cirrhosis have a certain degree of injury; Sacchromyces boulardii can help to protect intestinal mucosal barrier from injury by cirrhosis through inhibiting bacterial translocation and intestinal endotoxemia,thus may be considered as an adjunctive therapy in the treatment of chronic liver disease.