Biomarcadores histológicos e fisiológicos para o monitoramento da saúde de peixes de ribeirões de Londrina, Estado do Paraná

Two fish species, known as lambaris ( Astyanax altiparannae and A. fasciatus ) were collected from the following streams in Londrina: Cambe (lake Igapo IV), Tres Bocas, Apertados and Capivara. After capture blood samples and gills were immediately removed. Gills were fixed in Bouin and processed for light microscopy. A small amount of blood was used for hemoglobin determination and sodium and glucose plasma concentrations were determined after blood centrifugation. Animals from Cambe and Capivara streams showed certain severe histopathological alterations, such as lamellar aneurism and epithelial lifting. These fish also showed higher blood sodium concentration and a reduction of blood glucose when compared to fish from Tres Bocas stream. Fish from Capivara showed a decrease in hemoglobin content when compared to animals from Cambe stream. Higher incidence of histological and physiological alterations observed in fish from Cambe and Capivara stream clearly indicates the animals’ health impairment