T. Fuse, Takuji Sasaki, M. Yano
2001.9.1Plant Biotechnology
tlooto Summary
These binary vectors contain multiple cloning sites and the hygromycin B phosphotransferase gene as a plant-selectable marker to develop new binary vectors, pPZP2H-lac and pP ZP2Ha3, for the transformation of rice plants.
Abstract
We developed new binary vectors, pPZP2H-lac and pPZP2Ha3, for the transformation of rice plants. These binary vectors contain multiple cloning sites and the hygromycin B phosphotransferase gene as a plant-selectable marker. After Agrobacterium-mediated transformation, transgenic plants containing a T-DNA could be tightly selected by hygromycin B. The vectors are powerful tools for the functional analysis of genes.
Citation format
FUSE, T.; SASAKI, Takuji; YANO, M. Ti-plasmid vectors useful for functional analysis of rice genes. Plant Biotechnology, 2001, 18: 219–222.