ANTIOXIDATIVE ACTIVITY OF ETHANOL EXTRACTS FROM Spirulina platensis AND Nostoc linckia MEASURED BY VARIOUS METHODS

The goal of this work is to determine the level of antioxidative activity of various ethanol extracts from Spirulina platensis and Nostoc linckia biomass, and also to demonstrate the possibility to select suitable methods for evaluation of these characteristics. The methods for determination of antioxidative activity were selected concerning their possible use for complex preparations: phosphomolybdenum method for evaluation of antioxidant capacity (PMRC), radical-scavenging activity by DPPH method (DPPH), antioxidant activity by the ABTS + radical cation assay (ABTS), Folin-Ciocalteu reducing capacity (FCRC). We showed the presence of antioxidative substances in ethanol extractions from 2 species of cyanobacteria, and possibility to increase their activity varying ethanol concentration. It facilitates the extraction both water- and lipid-soluble components from biomass. Regarding used methods for antioxidative activity determination, we have used only those based on reaction of electrons return (which widely used nowadays in vitro). Obtained in different ways results demonstrate high reduction capacity of the extracts and possibility to select suitable analytical methods for each case.