Mutation of tumor-suppressor OVCA1 A34D extends the half-life of the protein

Objective To explore the relationship between the structure and function of tumor suppressor OVCA1,in order to clarify the mechanism of OVCA1 in tumorigenesis and progression.Methods The GFP-tagged-OVCA1A34 Dmutant was built by gene cloning and was confirmed by sequencing.The mutant was transfected into cells with liposome assay.The expression of OVCA1A34 Dmutant protein was detected and the half-life of the protein was measured by inhibiting protein synthesis with cycloheximide(CHX).Results GFP-tagged-OVCA1 A34 D was established successfully and was expressed in cells.The half-life of OVCA1A34 Dmutant protein was longer compared with that of wild type OVCA1 protein.Conclusion OVCA1A34 Dmutation extends the protein′s half-life.