Quantitative Determination of Fumonisins B1 and B2 by High-Performance Liquid Chromatography with Fluorescence Detection

Abstract A high-performance liquid chromatographic method has been developed for the quantitative determination of the recently described mycotoxins, fumonisins B1 (FB1) and B2 (FB2) utilizing pre-column derivatization with o-phthaldialdehyde, isocratic elution, and fluorescence detection. Prior to analysis, sample extracts were purified on strong anion exchange cartridges. The method has been applied to the analysis of naturally contaminated corn and mixed horse feed samples as well as fungal culture material, for the presence of the mycotoxins. Detection limits are approximately 50 ng g−1 for FB1 and 100 ng g−1 for FB2. The method proved to be highly reproducible and recoveries of the toxins from the purification steps were found to be 99.5% and 85.9% for FB1 and FB2, respectively.