Separation and Determination of Madecassic Acid in Extracts of Centella asiatica Using High Performance Liquid Chromatography with β-Cyclodextrin as Mobile Phase Additive

A method using reversed-phase high performance liquid chromatography in which β-cyclodextrin (β-CD) was the additive in mobile phase has been developed for separation and determination of the structural isomers of Centella asiatica and was successfully used to determine the madecassic acid in triterpenic genins.