Optimization of Extraction Process of Qinghou Liyan Buccal Tablets by Response Surface Methodology

Objective: To optimize extraction process of Qinghou Liyan buccal tablets,and provide references for its pilot production. Method: Based on pre-experiments,effects of soaking time,solvent dosage and extracting time on overall desibirability of contents of chlorogenic acid and totale flavonoids( included rutin,liquiritin and luteolin-7-O-glucoside) were investigated and forecasted by response surface metholodgy. HPLC was used to determine contents of chlorogenic acid and three flavonoids with mobile phase of acetonitrile-0. 5%phosphoric acid for gradient elution and detection wavelengthes of 327 nm and 256 nm. Result: Optimal extraction process was as following: soaked 89 minutes and extracted twice with 15. 3 times the amout of water for per time of136 minutes. Concentrations of chlorogenic acid,luteolin-7-O-glucoside,rutin and liquiritin were 1 843. 537,184. 845,93. 939,48. 898 mg·L~(-1). Conclusion: This opimized extraction technology is stable and feasible with well prediction,it provides references for pilot production of Qinghou Liyan buccal tablets.