Chemistry

M. Szabo, C. Idiţoiu, D. Chambre, A. Lupea

2007.6.1CHEMICAL PAPERS

DOI: 10.2478/s11696-007-0022-7

tlooto Summary

An improved procedure for DPPH determination was proposed to accurately assess antioxidant activity spectrophotometric assay.

Abstract

An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine) stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 % were found.

Citation format

SZABO, M., et al. Improved DPPH determination for antioxidant activity spectrophotometric assay. CHEMICAL PAPERS, 2007, 61: 214–216.