Surface Functionalization of TiO2 with Plant Extracts and their Combined Antimicrobial Activities Against E. faecalis and E. Coli

Abstract : The aim of this study is to enhance the antibacterial activity of TiO 2 by pure plant extracts of Bauhinia variegata and Tinospora. cordifolia by making a composite of plant extract and TiO 2 . Plant extracts, TiO 2 and plant extracts/TiO 2 composites were investigated against two bacterial strain Enterococcus faecalis and Escherichia coli . X-ray diffraction investigations have confirmed the presence of TiO 2 nanoparticles in the plant extract/TiO 2 nanocomposites. UV-visible investigations have shown an enhanced photocatalytic activity of plant extract/TiO 2 nanocomposites compared to that of pure TiO 2 and pure plant extract. Plant extract/TiO 2 nanocomposites have shown various level of antibacterial activity on different test microorganisms. The highest antibacterial potentiality expressed in terms of zone of inhibition (ZOI) in mm was exhibited by the aqueous extract of Bauhinia variegata /TiO 2 (45 mm against Enterococcus faecalis and 30 mm against Escherichia coli ) and benzene extract of Tinospora cordifolia /TiO 2 (26 mm) nanocomposites. This is the first study on these types of bio-nano composite materials and it serves as basis for further research on these types of composite materials as a potent antibacterial agent.