Influence of Dexamethasone on Blood TNF-α, IL-10, GR in Rats Models of Acute Necrotizing Pancreatitis

Objective To investigate the changes of TNF-α,IL-10,glucocorticoid receptor(GR) in Rats with acute necrotizing pancreatitis(ANP) and the influence of Dexamethasone(DEX) on these parameters in ANP rats.Methods Fourty-eight male Sprague-Dawley(SD) rats were randomly divided into acute-necrotizing-pancreatitis group(group A,n=16),DEX-treated group(group D,n=16) and sham-operation group(group S,n=16).ANP model was induced by intergraded injection of 5% sodium tanrocholate to biliopancreatic duct in the rats except for group S,and the rats in group D were intramus-cularly given dexamethasone after operation.The change of pancreatic pathology,the serum AMY,TNF-α and IL-10 level,and the blood mRNA expression of GR were examined.Results ① Typical ANP pathological changes were found in group A,including i.e.interstitial edema,infiltration of inflam-matory cells,hemorrhage and parenchyma necrosis;and the mild inflammation was found in rats of D group which treated by Dexamethasone.②The concentration of serum TNF-α,IL-10 in group A were obviously higher than that in group S(P0.01).The concentration of serum TNF-α decreasing and IL-10 increasing in rats were induced by the usage of Dexamethasone(P0.01).③The mRNA expression of blood GR in group D was lower significantly at 8h or 12h after the operation than that in group S(P0.01 or P0.05).Conclusions The changes of TNF-α,IL-10,GR are involved in the inflammation response of rat ANP,and the dexamethasone can relieve ANP inflammation response by affecting these factors.