The changes of nitric oxide, NO synthase and sFas/APO-1 in serum among the patients with Kashin-Beck disease

Objective To investigate the induced effect of nitric oxide, NO synthase, and sFas/APO-1 in serum in the pathogenesis of Kashin-Beck disease(KBD) in order to probe a new strategy to control the disease. Methods 120 subjects, from KBD areas of YongShou and YuYang counties, and non-KBD areas of XianYang city, ShaanXi province, were divided into 6 groups:(1) the adult group with KBD, (2) the child group with KBD, (3) the adult control in KBD areas, (4) the child control in KBD areas, (5) the adult control in no-KBD areas, (6) the child control in no-KBD areas. The sex and the average age in the different groups were not different. The contents of nitric oxide, NO synthase, and sFas/APO-1 in serum of 6 groups were detected by method of nitrifying ferment, chemic colorimetry and ELISA, respectively. Results ① The contents of NO and iNOS in serum of the adult group with KBD were significantly higher than those of the adult control in KBD areas and no-KBD areas(P 0.05). The contents of NOS and sFas/APO-1 in serum of the adult group with KBD were significantly higher than those of the adult control group in no-KBD areas (P 0.005). ② The contents of NO in serum of the child KBD group were significantly higher than those of the child control in no-KBD areas (P 0.05). The contents of NO and iNOS in serum of the child group with KBD were significantly higher than those of the child control group in KBD and no-KBD areas (P 0.005). Conclusion NO induced way may plays a role in the pathogenesis in KBD children, but not sFas, and both NO and Fas induced ways have some effects in the KBD development with age.