Effects of Bujingyishipian on the expression of retinal p-Akt in rat model of chronic elevated intraocular pressure

Objective To observe the effects of bujingyishipian on retinal p-Akt in SD rat model of chronic elevated intraocular pressure(EIOP),and explore the mechanism of it initially.Methods Thirty SD rats were randomly and equally divided into 3 groups:control group,model group and treatment group,10 rats in each group.By unilaterally cauterizing 3 episcleral vessels,the rat model of chronic EIOP was established in model group and treatment group.After given bujingyishipian(1.8 g·kg~(-1),once per day) or normal saline for 8 weeks,the rats were sacrificed.The intraocular pressure(IOP),expression of retinal p-Akt were observed.Results At models building and 8 weeks after-models,IOP was obviously increased by unilaterally cauterizing episcleral vessels(all P 0.01);IOP was obviously reduced at 8 weeks after-models in treatment group compared models building(P 0.01),while model group was not statistically significant(P 0.05).At 8 weeks after-models,the immunohistochemical analysis of retinal p-Akt expression showed that the total area in treatment group was(74 631.81 ± 12 841.30) μm~2,mean optical density was 939.26 ± 175.17,integrated optical density was 37 814.96 ± 5822.71,while the total area in model group was(37 947.26 ± 8050.51) μm~2,mean optical density was 481.45± 161.02,integrated optical density was 18 907.64±4367.29,there were statistical differences between two groups(all P 0.01).Though mean black degree in treatment group(138.55 ± 8.58)was little higher than model group(136.52±3.81),there was no significant difference in statistics(P0.05).Conclusion Bujingyishipian can protect the visual function on the rat model of chronic EIOP by up-regulating the expression of retinal p-Akt and reducing IOP slightly.