Effects of hyperbaric oxygen on the content of interleukin-1β and tumor necrosis factor-α in brain injured tissue of rats

Objective To observe the changes of content of interleukin-1β(IL-1β)and tumor necrosis factor-or(TNF-α)before and after hyperbaric oxygen(HBO)treatment in brain injured tissue of SD rats following severe lateral fluid-percussive brain injury and to explore the mechanism of HBO treatment on traumatic brain injury.Methods Severe traumatic brain injury models were established by the fluid percussion device.The concentrations of IL-1βand TNF-αin the homogenate of brain tissue were measured by using enzyme-linked immunosorbent assay(ELISA)respectively.Results The concentration of IL-1βand TNF-αin brain tissue homogenate in the HBO group was significantly lower than that of the traumatic brain injury group (P0.05 ).Conclusions HBO treatment can effectively relieve the inflammatory reaction of brain injured tissue in rats through inhibiting the expression of IL-1βand TNF-α.