Biology

C. Ono, Tomonori Nakatsukasa, Yasuyuki Nishijima, S. Asano, K. Sahara, H. Bando

2007.10.30Journal of Insect Biotechnology and Sericology

DOI: 10.11416/jibs.76.3_161

tlooto Summary

The BmNPV/T3 bacmid system constructed here is useful for the functional analysis of BnN cells and he65 is not essential for Bm NPV replication but critical for an optimal replication in BmN cells.

Abstract

-knockout BT3Bac (BT3Bac +polh -he65KO) compared to those in BT3Bac +polh or BmNPV/T3, indicating that he65 is not essential for BmNPV replication but critical for an optimal replication in BmN cells. The BmNPV/T3 bacmid system constructed here is useful for the functional analysis of BmNPV genes.

Citation format

ONO, C., et al. Construction of the bmnpv t3 bacmid system and its application to the functional analysis of bmnpv he65. Journal of Insect Biotechnology and Sericology, 2007, 76: 161–167.