Fully automated synthesis of 16α-[18F]fluoro-17-βestrogen using Explora GN/LC dual module

Objective To-synthesize 16a- [ ^18 F ] fluoro-17β-estrogen （ ^18 F-FES） with high chemical and radiochemical purity using multifunctional radiolabeling module of Explora GN and separation module of Explora LC （Explora GN/LC dual module）. Methods Explora GN/LC dual module and ＂one-pot＂ radiochemical process were applied for fully automated synthesis and separation of ^18F-FES. The radiochemical purity of ^18F-FES was analyzed by HPLC. The saturation binding test of ^18F-FES with ER （ER-positive MCF-7 cell line） was applied to examine the biological activity of 18 F-FES. Results is F-FES synthesis procedure took 60 min, with radiochemical yield about 45% and radiochemical purity higher than 98%. The Kd and Bmax of MCF-7 cells with is F-FES were （2. 922 ± 0.619） nmol/L and （4. 193 ± 0. 360） nmol/L respectively. Conclusions 18 F-FES can be automatically synthesized by Explora GN/LC dual module with high chemical purity and radioehemieal purity. It would be a potential PET tracer to be used in estrogen-relatod molecular imaging. Key words: Estradiol;  Fluorine radioisotopes;  Chemical synthesis ;  Automation