Glucose biosensor based on self assembled-in situ polymerized polyaniline /CNTs nanocomposite modified interdigitalelectrode

Glucose biosensors based on self assembled-in situ polymerized polyaniline /CNTs nanocomposite modified interdigitalelectrode were prepared. Firstly, CNTs modified interdigital electrode was prepared by self assembling process, and PANI/CNTs was prepared using p-toluene sulfonic acid doped polyaniline in situ polymerized aniline on the electrode surface; then glucose biosensor was prepared by immobilization of glucose oxidase on the as prepared modified electrode surface. The PANI/CNTs nanocomposites were characterized by Raman spectra analysis, scanning electron microscopy and atomic force microscopy; electrochemical behaviors of the modified electrode were characterized by cyclic voltammetry and electrochemical impedance method. The results showed that under the optimum conditions, glucose concentration and the response current showed a good linear relationship in the range of 0.5-30 mmol/L with the response sensitivity of 62.17 μA/(mmol·L-1), the linear correlation coefficient of 0.997, and the detection limit of 0.15 mmol/L(S/N=3). The biosensor also showed good stability and reproducibility.