E. Capo, C. Giguet-Covex, A. Rouillard, K. Nota, P. Heintzman, A. Vuillemin, D. Arizteguí, F. Arnaud, S. Belle, S. Bertilsson, C. Bigler, R. Bindler, Antony G. Brown, Charlotte L. Clarke, S. Crump, D. Debroas, G. Englund, G. Ficetola, Rebecca E. Garner, J. Gauthier, I. Gregory‐Eaves, Liv Heinecke, U. Herzschuh, Anan Ibrahim, V. Kisand, K. Kjær, Y. Lammers, J. Littlefair, E. Messager, Marie‐Eve Monchamp, Fredrik Olajos, W. Orsi, M. W. Pedersen, D. P. Rijal, J. Rydberg, Trisha L. Spanbauer, K. Stoof-Leichsenring, P. Taberlet, L. Talas, Camille Thomas, D. Walsh, Yucheng Wang, E. Willerslev, A. V. van Woerkom, H. Zimmermann, M. Coolen, L. Epp, I. Domaizon, Inger G. Alsos, L. Parducci
2021.2.13Quaternary
tlooto Summary
Building on the most recent literature and seven original case studies, the state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation of DNA inventories from sedimentary ancient DNA via high-throughput sequencing technologies are synthesized.
Abstract
The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays, knowledge gaps remain in this field and there is therefore still research to be conducted to ensure the reliability of the sedimentary DNA signal. Building on the most recent literature and seven original case studies, we synthesize the state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation of DNA inventories from sedimentary ancient DNA (sedaDNA) via high-throughput sequencing technologies. We provide recommendations based on current knowledge and best practises.
Citation format
CAPO, E., et al. Lake sedimentary DNA research on past terrestrial and aquatic biodiversity: Overview and recommendations. Quaternary, 2021.